How do substrate concentration and enzyme concentration affect enzyme activity?
Initially, an increase in substrate concentration leads to an increase in the rate of an enzyme-catalyzed reaction. As the enzyme molecules become saturated with substrate, this increase in reaction rate levels off. The rate of an enzyme-catalyzed reaction increases with an increase in the concentration of an enzyme.
What is a Michaelis Menten graph?
A graph that shows the relationship between the concentration of a substrate and the rate of the corresponding enzyme-controlled reaction.
How do you graph enzyme activity?
When plotting line graphs for enzyme rate experiments, remember the following:
- Plot data points accurately.
- Use appropriate linear scales on axes.
- Choose scales that enable all data points to be plotted within the graph area.
- Label axes, with units included.
- Make graphs that fill the space the exam paper gives you.
What does a Lineweaver graph show?
The Lineweaver Burk plot is a graphical representation of enzyme kinetics. The x-axis is the reciprocal of the substrate concentration, or 1 / [S], and the y-axis is the reciprocal of the reaction velocity, or 1 / V. In this way, the Lineweaver Burk plot is often also called the double reciprocal plot.
How do you graph Lineweaver-Burk equation?
Lineweaver-Burk plot. This equation can be compared with the equation for a straight line: y = mx + b, where m is the slope and b is the y-intercept. In the Lineweaver-Burk equation, Km/Vmax is the slope (or m) and 1/Vmax is the y-intercept (or b).
How do you plot a graph Michaelis-Menten?
Using graph paper, draw an x- and y-axis. Label the x-axis mM of [S] or concentration of substrate. Label the y ax- sec/micro-mole of V or velocity of reaction. Insert different values of [S] into the Michaelis-Menten equation, along with the values found for Km and Vmax, to solve for V.
What is Lineweaver Burk plot of enzyme kinetics?
What does Lineweaver Burk plot?
The Lineweaver–Burk plot was widely used to determine important terms in enzyme kinetics, such as Km and Vmax, before the wide availability of powerful computers and non-linear regression software. The y-intercept of such a graph is equivalent to the inverse of Vmax; the x-intercept of the graph represents −1/Km.
What does the slope of a Lineweaver Burk plot represent?
On the graph, the x-intercept can be used to determine the Michaelis constant, the y-intercept can be used to determine the maximal velocity and the slope represents the ratio of the Michaelis constant to the maximal velocity.
Why is Lineweaver Burk plot used?
Uses of Lineweaver–Burk Plot Used to determine important terms in enzyme kinetics, such as Kmand Vmax, before the wide availability of powerful computers and non-linear regression software. Gives a quick, visual impression of the different forms of enzyme inhibition.