How long is PMSF stable?

Stock solutions of PMSF in 100% isopropanol are stable for at least 9 months at 15 to 25 °C.

What is PMSF for in lysis buffer?

Product Description. Phenylmethanesulfonyl Fluoride (PMSF) is an inhibitor of serine proteases such as trypsin, chymotrypsin, thrombin, and papain. It is routinely added as a supplement to lysis buffers just prior to lysis, to prevent protease degradation.

What is PMSF in protein extraction?

PMSF (Phenylmethylsulfonyl fluoride) Acting as a non-specific inhibitor, PMSF is often used to protect samples against the digestive functions of proteases, such as trypsin, chymotrypsin, and thrombin, as well as other enzymes. It is also used when preparing protein extracts taken from cells and tissues.

How do you handle PMSF?

Keep containers tightly closed. PMSF may corrode/etch glass; therefore do not store aqueous solutions of PMSF in glass containers. Store in a cool, dry, and well-ventilated area away from incompatible chemicals such as aqueous solutions, strong oxidizing agents, strong bases, and acids.

Is PMSF reversible?

Inhibition of the cysteine proteases by PMSF or AEBSF can be reversed by treatment with a sulfhydryl reagent such as DTT, but inhibition of serine proteases is irreversible.

How does PMSF inhibit protease?

PMSF works by deactivating the serine hydroxyl group and any other enzyme that contains serine in its active site through an esterification process.

Is PMSF a phosphatase inhibitor?

It inhibits many tyrosine phosphatases, acid phosphatases, and alkaline phosphatases. PMSF (100 mM) is used for inhibiting serine proteases, including tryptase, chymotrypsin, callicrein, subtilisin, and thrombin. It also inhibits the cysteine protease papain.

How do I prepare for PMSF?

How to make a 1 M PMSF Stock Solution

1. Weigh 1.74 g PMSF. 2 g. 00:03:00. PMSFP212121Catalog #RP-P20270.
2. Add DMSO to a final volume of 100 ml. Dissolve completely. 00:03:00.
3. Stocks may be kept at -20°C for up to 6 months. PMSF is used at a final concentration of 0.1- 1.0 mM. PMSFP212121Catalog #RP-P20270.

Where is PMSF stored?

Store at −20°C. PMSF is inactivated in aqueous solutions. The rate of inactivation increases with increasing pH and is faster at 25°C than at 4°C. The half-life of a 20 mM aqueous solution of PMSF is ~35 min at pH 8.0.

Is PMSF soluble in water?

Use. PMSF is not soluble in water, dissolve in isopropanol, ethanol or methanol. 200 mM stock solution (0.035 g/mL) is stable for months at 4°C, although storage at -20 is probably better. A common final concentration of PMSF is 1mM.

What type of inhibitor is PMSF?

protease inhibitor
Thermo Scientific PMSF is a protease inhibitor that reacts with serine residues to inhibit trypsin, chymotrypsin, thrombin and papain. PMSF is an abbreviation for phenylmethylsulfonyl fluoride, the most common chemical name for this small compound.

How do you prepare PMSF stock solution?

How much PMSF do I add?

To prepare a 100 mM solution, add 17.4 mg of PMSF per milliliter of isopropanol. Store at −20°C. PMSF is inactivated in aqueous solutions. The rate of inactivation increases with increasing pH and is faster at 25°C than at 4°C.

What is PMSF soluble in?

PMSF is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF), which should be purged with an inert gas. The solubility of PMSF in ethanol and DMSO is approximately 20 mg/ml and approximately 33 mg/ml in DMF.

How do you mix PMSF?

In which solvent would you dissolve PMSF and why?

PMSF is considered a highly toxic cholinesterase inhibitor. This product is soluble at 0.2 M (200 mM) in isopropanol. When needed, sonication (or gentle heating, if absolutely necessary) may be applied to dissolve fully the PMSF.