What is cytospin centrifugation?

A cytocentrifuge, sometimes referred to as a cytospin, is a specialized centrifuge used to concentrate cells in fluid specimens onto a microscope slide so that they can be stained and examined.

What is the cytospin technique?

The cytospin technique uses a high speed centrifuge to concentrate the cells on a slide in a uniform monolayer 6 mm in diameter. The monolayer distribution enhances the morphological appearance of the cells present. Allow the slides to dry in air for several minutes and then stain them with Wright-Giemsa stain.

What is the meaning of cytospin?

cytospin (plural cytospins) A form of centrifuge used to prepare cell samples for microscopy.

Why do you centrifuge or cytospin a body fluid specimen?

Preparation of body fluid smears for microscopic examination requires concentration techniques that preserve cell integrity and morphology. Cytocentrifugation (also known as “cytospin”) provides the best method for both concentrating cells in body fluid samples and maintaining cellular morphology.

Why is a cytospin used?

A cytospin is obtained by employing centrifugal force to isolate, concentrate and deposit a monolayer of cells from a dilute cell suspension onto a circular area on a slide. The objective is to keep cells intact enabling the morphology of the cells to be examined.

What is cytospin 4 centrifuge?

Gain all the advantages of the ultimate thin–layer cell preparation system with the Epredia™ Cytospin™ 4 Cytocentrifuge. This reliable benchtop centrifuge provides economical thin–layer preparations from any liquid matrix, especially hypocellular fluids such as spinal fluid and urine.

How to optimize the loading volume of the cytospin?

The loading volume should be optimized for each cell line used. Close the Cytospin lid and centrifuge at 1000 × g for 5 min. Remove the slides, filter cards, and cytofunnels from the centrifuge. Be careful not to move the filter paper on the slide and so damage/smudge the cell preparation.

What is the cytospin used for?

• There are many published technical papers utilizing the Cytospin for the rapid detection of respiratory viruses, CMV Detection, ISH, FISH, etc. Research/General Use

How do you get a Cytospin from cells?

A cytospin is obtained by employing centrifugal force to isolate, concentrate and deposit a monolayer of cells from a dilute cell suspension onto a circular area on a slide. The objective is to keep cells intact enabling the morphology of the cells to be examined.