What media do CHO cells use?
CHO cells should be cultured in Ham’s F12K (ATCC suggestion) or DMEM modified with 10% FBS. If cells are not doubling every 14-17 hours, supplement the medium with 1-2% FCS. Subculture Protocol for CHO: CHO cells grow quickly and easily and cell count should have a doubled within 14-17 hours.
What type of cells are CHO?
Chinese hamster ovary (CHO) cells are an epithelial cell line derived from the ovary of the Chinese hamster, often used in biological and medical research and commercially in the production of recombinant therapeutic proteins.
What is the difference between CHO and CHO-K1?
both are derived from CHO-original cell line. CHO-K1 suggests that this cell line is missing a chromosome which carries a gene necessary for glycine biosynthesis. whereas, CHO-S adapted for growth in suspension liquid culture, this cell line is ideal for scale up and growth in large-scale bioreactors.
Why are CHO-K1 cells used?
The Advantages of CHO Cells Grow well in suspension and as adherent culture, rendering the cells ideal for GMP procedures. Their tolerance to variations in pH, oxygen levels, temperature or pressure make them the ideal cell for large-scale culture. High recombinant protein yields and specific productivity.
What is CHO media?
Chinese Hamster Ovary (CHO) Cell Media. Chinese Hamster Ovary (CHO) cells are of great interest for bioprocessing and pharmaceutical research and development. These cells are robust in culture and are able to produce a variety of recombinant glycoproteins at high levels on a large scale.
What is in DMEM media?
DMEM is a modification of Basal Medium Eagle (BME) that contains four fold concentration of amino acids and vitamins. Additionally, the formulation also includes glycine, serine and ferric nitrate. The original formulation contains 1000mgs/L of glucose and was originally used to culture embryonic mouse cells.
What are CHO cells and what are they used for?
CHO cells, which are a cell line derived from the ovary of the Chinese hamster, meant for use in biological and medical research and commercially in the production of therapeutic proteins.
What is CHO K1 cell line?
CCL-61™ The CHO-K1 cell line was derived as a subclone from the parental CHO cell line, which was initiated from a biopsy of an ovary of an adult, female Chinese hamster in 1957. The cell line can be used for industrial biotechnology and toxicology research. Animal cells.
What are CHO GS cells?
The CHO-GS cell line is the prevalent protein expression system worldwide for therapeutic antibodies. CHO cell protein production laboratories are predominantly using serum-free CHO media.
What is DG44 CHO?
The dihydrofolate reductase-deficient Chinese hamster ovary (CHO) cell line DG44 is the dominant mammalian host for recombinant protein manufacturing, in large part because of the availability of a well-characterized genetic selection and amplification system.
What is GS CHO?
CHO-K1SV is a suspension, protein-free-adapted CHO-K1-derived cell line employing the glutamine synthetase (GS) gene expression system (GS-CHO expression system). The selection of high-producing mammalian cell lines is a crucial step in process development for the production of therapeutic antibodies.
What is serum free media?
Serum-free media are media designed to grow a specific cell type or perform a specific application in the absence of serum. The use of serum-free media (SFM) represents an important tool, that allows cell culture to be done with a defined set of conditions as free as possible of confounding variables.
What is the difference between DMEM and MEM?
DMEM is richer in a variety of nutrients than MEM and is a very good choice for many cell types. Cells will grow faster and deplete the medium slower in DMEM. You should also consider Opti-MEM which, in my experience, is even better than DMEM. HEK cells will grow fine and very similar at 5-10% FBS in both MEM and DMEM.
Why are CHO cells used for transfection?
From there, they are ideal for transfection technology: CHO cells have a high transfection efficiency rate compared with other mammalian expression systems. Can be transfected through viral means to introduce genomic DNA for target proteins.
Why are CHO cells important to the pharmaceutical industry?
CHO cells have manifold advantages for the production of complex pharmaceuticals like antibodies. A big asset of CHO cells is their robust growth and adaptability to different media and culture conditions.
What is MSX cell culture?
MSX, a drug similar to glutamate, binds to GS, thereby inhibiting the production of glutamine, which is necessary for cell to growth. During the gene amplification process, CHO cells are cultured in high levels of MSX. CHO cells that have increased copies of the GS gene, with the gene of interest, are selected.
Are CHO cells adherent or suspension?
Fact #7 – CHO cells are adherent … and so much else Such cell types can be subcultured by simply taking a small volume of the parent culture and diluting it in a fresh growth medium. But to get back to the heart of the matter: CHO cells are adherent and can also be grown in suspension.
What is GS Cho?
What is the best media for growth of CHO cells?
IMDM with 10% FBS is a very good media which supports growth and maintains the morphology of CHO cell intact. Its having a good buffering effect becoz of sodiumbicarbonate and HEPES. If you want chemically defined and protein free media Invitrogen is supplying so many.
What is a CHO cell line?
These CHO cell lines are, however, immortalized cells, each representing different cell types, based on their inherent genetic diversity and their dynamic rate of genetic change.
What is the best way to culture CHO cells with FBS?
CHO cells should be cultured in Ham’s F12K , but you can try with RPMI-1640 IMDM with 10% FBS , alpa-MEM, and DMEM modified with 10% FBS. I am using DMEM with 10% FBS for CHO cells. Its good! Can you help by adding an answer?
What is the difference between Cho-s and CHO-K1 cells?
It was said that CHO-S cell line have been distinguished as a separate sub-clone from the common CHO-K1, and what are the connection and difference between CHO-S and CHO-K1? What is a suitable culture medium for CHO-K1 cells? First of all cells will be cultured on monolayer and than will be transformed to suspension.