How do you test for herpes in CSF?

Detection of herpes simplex virus (HSV) DNA in cerebrospinal fluid (CSF) using PCR assay has been validated for the diagnosis of central nervous system (CNS) herpes infections.

How accurate is PCR test for HSV?

PCR assays or other NAATs are the most sensitive test currently available to detect HSV in clinical samples. The detection rates of the PCR assays were shown to be 11–71% superior to virus culture [26,41-44].

How long is HSV PCR positive in CSF?

In conclusion, we report the persistence of a positive HSV DNA PCR in the CSF of four neonates following 15, 18, 20 and 21 days of high-dose acyclovir. All of the infants had abnormal neuroimaging studies and subsequently experienced severe developmental delay or death.

Can you be tested negative for herpes and still have it?

A negative (normal) test most often means you have not been infected with HSV-1 or HSV-2. If the infection occurred very recently (within a few weeks to 3 months), the test may be negative, but you may still be infected. This is called a false negative.

Can a HSV PCR test be wrong?

Variations in quality. In a recent study of 27 coded samples tested by 66 labs, 30 false positive HSV PCR results were reported, as well as 30-50% false negative results for samples containing low titers of 0.2-7 copies/µL. Method used in the Virology Laboratory.

Can a HSV swab test be false positive?

The herpes culture test (swab test) has a 0% chance of a false-positive test result. This means that a person who tests positive can be 100% sure that they have the herpes infection. The commercially available herpes IgG test (HerpeSelect) has up to a 19% probability of revealing false-positive test results.

Can you transmit herpes when it’s dormant?

Herpes dormancy period Once a person has contracted HSV, they can transmit the virus even during dormant periods when there are no visible sores or other symptoms. The risk of transmitting the virus when it’s dormant is less. But it’s still a risk, even for people who are receiving treatment for HSV.