How do you use ocular and stage micrometers?
Place a stage micrometer on the microscope stage, and using the lowest magnification (4X), focus on the grid of the stage micrometer. Rotate the ocular micrometer by turning the appropriate eyepiece. Move the stage until you superimpose the lines of the ocular micrometer upon those of the stage micrometer.
What is the purpose of using an ocular micrometer?
An ocular micrometer is a glass disk that fits in a microscope eyepiece that has a ruled scale, which is used to measure the size of magnified objects. The physical length of the marks on the scale depends on the degree of magnification.
What is stage micrometer?
A stage micrometer is simply a microscope slide with a scale etched on the surface. A typical micrometer scale is 2 mm long and at least part of it should be etched with divisions of 0.01 mm (10 µm). Suppose that a stage micrometer scale has divisions that are equal to 0.1 mm, which is 100 micrometers (µm).
What is ocular unit?
An ocular unit is the space between the ocular micrometer that is filled up by the stage micrometer that has lines that are 10 micrometers apart. It allows scientists to communicate the true size of their specimen regardless of the objective lens in use by standardizing 1 ocular unit to the specific true size observed.
Where is the ocular micrometer placed?
The ocular micrometer is placed in one of the eyepieces of the microscope; however, the distance between the etched lines depends upon the objective lens used to view the specimen.
Where is the stage micrometer found?
In the example above, the eyepiece micrometer (reticle image) is on the top and the stage micrometer image is on the bottom. The stage micrometer is 1 mm long with 100 divisions so each division of the stage micrometer is one one-hundredth of a mm (0.01mm or 10 um).
How is the stage micrometer different from the ocular micrometer quizlet?
Ocular micrometers consist of a circular disk of glass that has graduations engraved on its upper surface, while stage micrometers have lines inscribed on it that are exactly 0.01 (10 micrometers) mm apart.
What is ocular microscope?
The eyepiece, or ocular lens, is the part of the microscope that magnifies the image produced by the microscope’s objective so that it can be seen by the human eye.
What is the function of stage?
The stage serves as a space for actors or performers and a focal point (the screen in cinema theaters) for the audience. As an architectural feature, the stage may consist of a platform (often raised) or series of platforms.
What is an ocular division?
The spaces on the ocular micrometer are called ocular units and the spaces on the stage micrometer are called stage units. Divide the number of stage units by the number of ocular units. For example, if you count 21 stage units and 29 ocular units, dividing these numbers gives 0.724.
How do the graduations differ between ocular and stage micrometer?
How do the graduations differ between ocular and stage micrometers? Ocular micrometers consist of a circular disk of glass that has graduations engraved on its upper surface, while stage micrometers have lines inscribed on it that are exactly 0.01 (10 micrometers) mm apart.
What is stage microscope?
All microscopes are designed to include a stage where the specimen (usually mounted onto a glass slide) is placed for observation. Stages are often equipped with a mechanical device that holds the specimen slide in place and can smoothly translate the slide back and forth as well as from side to side.
Where is the ocular lens?
While an objective is on the side of the observed object, the ocular lens (also called ocular or eyepiece, sometimes loupe) is on the side of the observing eye. It may contain a single optical lens or some combination of lenses and is normally placed in a cylindrical housing (barrel).
What are ocular units?
One ocular unit is the smallest unit visible on the scale. There are 100 of these in total. Using the mechanical stage controls, line up the left-hand (zero) end of the ocular scale with the left-hand (zero) end of the 2 mm stage scale.
How do you superimpose ocular and stage micrometers?
Move the stage until you superimpose the lines of the ocular micrometer upon those of the stage micrometer. With the lines of the 2 micrometers coinciding at one end of the field, count the spaces of each micrometer to a point at which the lines of the micrometers coincide again.
How many ocular micrometer graduations fit between two stage micrometers?
Figure 2-3 indicates that six ocular micrometer graduations fit between two stage micrometer graduations; therefore, one space of the ocular micrometer is equal to 10 µm/6 or 1.66 µm.
What is an ocular micrometer?
An ocular micrometer (Fig. 2-1) is a glass disc on which a series of uniformly spaced lines has been inscribed. The ocular micrometer is placed in one of the eyepieces of the microscope; however, the distance between the etched lines depends upon the objective lens used to view the specimen.
What is a microscope stage micrometer?
Stage micrometer: The measurement on the stage of the microscope where an object is to be kept. This micrometer is of a slide’s shape and size and has a mount of very finely graduated scale.