What is HuH-7 cell line?

What is HuH-7 cell line?

HuH-7 (hereafter Huh7) is a permanent cell line established from male hepatoma tissue, which was surgically removed from a 57-year-old Japanese male in 1982 (Nakabayashi et al., 1982). Huh7 and its derivatives have been used as a convenient experimental substitute for primary hepatocytes.

How do you identify a cell line?

Cell lines can be tested and identified using multiallelic variable number of tandem repeats (VNTR). HLA typing and DNA fingerprinting using short tandem repeat (STR) and a variable number of tandem repeats for intra-species cross-contamination have been used for cell line identification.

How do you maintain a HuH-7 cell?

Renew medium 2-3 times a week, or as needed according to media pH, and maintain cell confluency between 30-90%. Do not allow cell concentration to exceed 90% confluency, otherwise cells will decrease proliferation rates. The doubling time of HuH-7 cells is 24 hours.

What are HuH-7 cells used for?

Huh-7 Origin and Sato, J. Huh-7 is highly susceptible to the hepatitis C virus (HCV), and it is often used as a model to investigate hepatoma and HCV. This cell line can be used for an HCV replicon system, which allows for an in vitro production of infectious HCV particles and development of drugs that combat HCV.

What is cell line hepatocellular carcinoma?

Hepatocellular carcinoma (HCC) cell lines are useful in vitro models for the study of primary HCCs. Because cell lines acquire additional mutations in culture, it is important to understand to what extent HCC cell lines retain the genetic landscapes of primary HCCs.

How do you authenticate a mouse cell line?

Unlike other providers, we authenticate cell lines by comparing the STR profile of sample cell lines with the ATCC Mouse STR Database. If your cell lines are not from ATCC or no matching profile is found, we can still conduct a comprehensive interpretation of results and provide an STR profile.

How do you thaw HuH-7 cells?

  1. Pre-warm growth medium in 37 °C water bath.
  2. Thaw the cells in a 37 °C water bath (not completely, there should be a small visible ice clump inside the tube)
  3. Transfer the cells into a 15 ml centrifuge tube containing 9 ml pre-warmed growth medium.
  4. 7.1.

What is HepG2 cell line?

Hep G2 [HEPG2] is a cell line exhibiting epithelial-like morphology that was isolated from a hepatocellular carcinoma of a 15-year-old, White, male youth with liver cancer. The cell line was deposited by the Wistar Institute and is a suitable transfection host.

What are mouse cells?

Mouse cell lines are established from mouse tissue that originates from liver, neural segments, blood, tumorigenic cells, and fibroblasts. The purpose of choosing a particular type of tissue is for disease or protein assessment, or retroviral vector production and cell culture support.

What is the size of a mouse cell?

The average diameter of 50 mesenchymal stem cells from the mouse was 28 (SD 0.86) µm and 50 from the whale was 29 (SD 0.71) µm. The difference in cell size between the species was not statistically significant.

How is a cancerous is different from normal cell?

How is a cancerous cell different from a normal cell?…

Cancer cells Normal cells
i. Divide in an uncontrolled manner. i. Divide in a regulated manner.
ii. Do not show contact inhibition. ii. Show contact inhibition.

How do you revive a frozen cell line?

Guidelines for thawing cells

  1. Thaw frozen cells rapidly (< 1 minute) in a 37°C water bath.
  2. Dilute the thawed cells slowly before you incubate them, using pre-warmed growth medium.
  3. Plate thawed cells at high density to optimize recovery.
  4. Always use proper aseptic technique and work in a laminar flow hood.

What is a Vero cell line?

The Vero cell line is an immortalized cell line established from kidney epithelial cells of the African green monkey. A variety of Vero sublines have been developed and can be classified into four major cell lineages.